Selected Publications


Nguyen, Anne, Elham Rouhollahi, Roland Böttger, Chun Yat Ong, Po-Han Chao, Jiamin Wu, Yao Chen and Shyh-Dar Li.



Pauli, Griffin, Po-Han Chao, Zhu Qin, Roland Böttger, Suen E. Lee, and Shyh-Dar Li. Liposomal Resiquimod for Enhanced Immunotherapy of Peritoneal Metastases of Colorectal Cancer. Pharmaceutics 13, no. 10: 1696.




Nojoud AL Fayez, Roland Böttger, Elham Rouhollahi, Pieter R. Cullis, Dominik Witzigmann, and Shyh-Dar Li. Improved Liver Delivery of Primaquine by Phospholipid-Free Small Unilamellar Vesicles with Reduced Hemolytic Toxicity. Molecular Pharmaceutics. doi: 10.1021/acs.molpharmaceut.1c00520

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Anne Nguyen, Roland Böttger, Shyh-Dar Li. Recent trends in bioresponsive linker technologies of Prodrug-Based Self-Assembling nanomaterials. Biomaterials. doi: 10.1016/j.biomaterials.2021.120955

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K. K. DurgaRao Viswanadham, Roland Böttger, Lukas Hohenwarter, Anne Nguyen, Elham Rouhollahi, Alexander Smith,Yi-Hsuan Tsai, Yuan-Yu Chang, Christopher Llynard Ortiz, Lee-Wei Yang, Liliana Jimenez, Siyuan Li, Chan Hur, and Shyh-Dar Li. An Effective and Safe Enkephalin Analog for Antinociception. Pharmaceutics. doi: 10.3390/pharmaceutics13070927


JulianVogler, RolandBöttger, Nojoud A.L.Fayez, Wunan Zhang, Zhu Qin, Lukas Hohenwarter, Po-Han Chao, Elham Rouhollahi, Nida Bilal, Naliangzi Chen, BrandonLee, ChristineChen, BraydenWilkinson, Timothy J. Kieffer, Jayesh A. Kulkarni, Pieter R. Cullis, Dominik Witzigmann, Shyh-DarLi. Altering the Intra-Liver Distribution of Phospholipid-Free Small Unilamellar Vesicles Using Temperature-Dependent Size-Tunability. Journal of Controlled Release. doi: 10.1016/j.jconrel.2021.03.025




Anne Nguyen, Hidenori Ando, Roland Böttger, K. K. DurgaRao Viswanadham, Elham Rouhollahi, Tatsuhiro Ishida and Shyh-Dar Li. Utilization of click chemistry to study the effect of poly(ethylene)glycol molecular weight on the self-assembly of PEGylated gambogic acid nanoparticles for the treatment of rheumatoid arthritis. Biomaterials Science. doi: 10.1039/D0BM00711K









Roland Böttger, GriffinPauli, Po-Han Chao, Nojoud AL Fayez, Lukas Hohenwarter, Shyh-Dar Li. Lipid-based nanoparticle technologies for liver targeting Advanced Drug Delivery Reviews. doi:10.1016/j.addr.2020.06.017


Zhang, Wunan; Böttger, Roland; Qin, Zhu; Kulkarni, Jayesh; Vogler, Julian; Cullis, Pieter; Li, Shyh-Dar. Phospholipid-Free Small Unilamellar Vesicles for Drug Targeting to Cells in the Liver.

Small. doi: 10.1002/smll.201901782


Hamano, Nobuhito; Böttger, Roland; Lee, Suen Ern; Yang, Yang; Kulkarni, Jayesh; Ip, Shell; Cullis, Pieter; Li, Shyh-Dar. A robust microfluidic technology and a new lipid composition for fabrication of curcumin-loaded liposomes: Effect on the anticancer activity and safety of cisplatin. Mol Pharm. 2019 Aug 5 doi: 10.1021/acs.molpharmaceut.9b00583.



Mahjub R, Jatana S, Lee SE, Qin Z, Pauli G, Soleimani M, Madadi S, Li SD. Recent advances in applying nanotechnologies for cancer immunotherapy. J Control Release. 2018 Sep 14:239-263. doi: 10.1016/j.jconrel.2018.09.010.


Agrawal M, Saraf S, Saraf S, Antimisiaris SG, Hamano N, Li SD, Chougule M, Shoyele SA, Gupta U, Ajazuddin, Alexander A. Recent advancements in the field of nanotechnology for the delivery of anti-Alzheimer drug in the brain region. Expert Opin Drug Deliv. 2018 May 17:1-29. doi: 10.1080/17425247.2018.1471058.


Tang WL, Tang WH, Li SD. Cancer theranostic applications of lipid-based nanoparticles. Drug Discov Today. 2018 May; 23(5): 1159-1166. doi: 10.1016/j.drudis.2018.04.007.


Tang WL, Tang WH, Szeitz A, Kulkarni J, Cullis P, Li SD. Systemic study of solvent-assisted active loading of gambogic acid into liposomes and its formulation optimization for improved delivery. Biomaterials. 2018 Jun; 166:13-26. doi: 10.1016/j.biomaterials.2018.03.004.


Ho L, Bokharaei M, Li SD. Current update of a thermosensitive liposomes composed of DPPC and Brij78. J Drug Target. 2018 Jan 11:1-41.




Chang CC, Yang Y, Gao DY, Cheng HT, Hoang B, Chao PH, Chen LH, Bteich J, Chiang T, Liu JY, Li SD, Chen Y. Docetaxel-carboxymethylcellulose nanoparticles ameliorate CCl4-induced hepatic fibrosis in mice. J Drug Target. 2017 Dec 27:1-9.


Hoang B, Ernsting MJ, Tang WS, Bteich J, Undzys E, Kiyota T, Li SD.Cabazitaxel-conjugated nanoparticles for docetaxel-resistant and bone metastatic prostate cancer. Cancer Lett. 2017 Dec 1;410:169-179.


Roy A, Zhao Y, Yang Y, Szeitz A, Klassen T, Li SD. Selective targeting and therapy of metastatic and multidrug resistant tumors using a long circulating podophyllotoxin nanoparticle. 2017 Aug;137:11-22. doi: 10.1016/j.biomaterials.2017.05.019


Tang WL, Tang WH, Chen WC, Diako C, Ross CF, Li SD. Development of a Rapidly Dissolvable Oral Pediatric Formulation for Mefloquine Using Liposomes. Mol Pharmaceutics. 2017 May 1 [Web]. doi: 10.1021/acs.molpharmaceut.7b00077


Hysi E, Wirtzfeld LA, May JP, Undzys E, Li SD, Kolios MC. Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation. Photoacoustics. 2017 Mar;5(2017):25-35.


Yang Y, Roy Aniruddha, Zhao Yucheng, Undzys E, Li SD. Comparison of Tumor Penetration of Podophyllotoxin–Carboxymethylcellulose Conjugates with Various Chemical Compositions in Tumor Spheroid Culture and In Vivo Solid Tumor. Bioconjugate Chem. 2017 Apr 24 [Web]. doi: 10.1021/acs.bioconjchem.7b00165




Yang Y, Bteich J, Li SD. Current Update of a Carboxymethylcellulose-PEG Conjugate Platform for Delivery of Insoluble Cytotoxic Agents to Tumors. AAPS J. 2016 Nov 21. [Epub ahead of print]

May JP, Hysi E, Wirtzfeld LA, Undzys E, Li SD, Kolios MC. Photoacoustic Imaging of Cancer Treatment Response: Early Detection of Therapeutic Effect from Thermosensitive Liposomes. PLoS One. 2016 Oct 27; 11(10):e0165345.


Roy A, Li SD. Modifying the tumor microenvironment using nanoparticle therapeutics. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2016 Apr 1.


Tang WL, Chen WC, Roy A, Undzys E, Li SD. A Simple and Improved Active Loading Method to Efficiently Encapsulate Staurosporine into Lipid-Based Nanoparticles for Enhanced Therapy of Multidrug Resistant Cancer. Pharm Res. 2016 May; 33(5):1104-14.


May JP, Undzys E, Roy A, Li SD. Synthesis of a Gemcitabine Prodrug for Remote Loading into Liposomes and Improved Therapeutic Effect. Bioconjug Chem. 2016 Jan 20; 27(1):226-37.





Roy A, Ernsting MJ, Undzys E, Li SD*. A highly tumor-targeted nanoparticle of podophyllotoxin penetrated tumor core and regressed multidrug resistant tumors. Biomaterials. 2015; 52:335-46.



Hoang B, Ernsting MJ, Roy A, Murakami M, Undzys E, Li SD*. Docetaxel-carboxymethylcellulose nanoparticles target cells via a SPARC and albumin dependent mechanism. Biomaterials. 2015; 59:66-76.


Zhao Y, May JP, Chen IW, Undzys E, Li SD*. A Study of Liposomal Formulations to Improve the Delivery of Aquated Cisplatin to a Multidrug Resistant Tumor. Pharm Res. 2015; 32(10):3261-8.

Ernsting MJ, Hoang B, Lohse I, Undzys E, Cao P, Do T, Gill B, Pintilie M, Hedley D, Li SD*. Targeting of metastasis-promoting tumor-associated fibroblasts and modulation of pancreatic tumor-associated stroma with a carboxymethylcellulose-docetaxel nanoparticle. J Control Release. 2015; 206:122-30.




Hoang B, Ernsting MJ, Murakami M, Undzys E, Li SD*. Docetaxel-carboxymethylcellulose nanoparticles display enhanced anti-tumor activity in murine models of castration-resistant prostate cancer. Int J Pharm 2014;471(1-2):224-33. (PDF)


Roy A, Murakami M, Ernsting MJ, Hoang B, Undzys E, Li SD*. Carboxymethylcellulose-Based and Docetaxel-Loaded Nanoparticles Circumvent P-Glycoprotein-Mediated Multidrug Resistance. Mol Pharm. 2014;11(8):2592-9 (PDF)

Roy A, Bhattacharyya M, Ernsting MJ, May JP, Li SD*. Recent progress in the development of polysaccharide conjugates of docetaxel and paclitaxel. WIREs Nanomedicine & Nanotechnology 2014; 6(4):349-68. (PDF)




May JP, Ernsting MJ, Undzys E, Li SD*. Thermosensitive liposomes for delivery of gemcitabine and oxaliplatin to tumors. Mol Pharm 2013; 10(12):4499-508.

Ernsting MJ, Murakami M, Roy A, Li SD*. Factors controlling pharmacokinetics, biodistribution and intratumoral penetration of nanoparticles. J Control Release 2013; 172(3): 782-794. (PDF)


Murakami M, Ernsting MJ, Undzys E, Holwell N, Foltz WD, Li SD*. Docetaxel conjugate nanoparticles that target α-smooth muscle actin expressing stromal cells suppress breast cancer metastasis. Cancer Research. 2013; 73(15): 4862-71. (PDF)


May JP, Li SD*. Hyperthermia induced drug targeting. Expert Opinion on Drug Delivery. 2013; 10(4): 511-27.

Chen WC, May JP, Li SD*. Immune responses of therapeutic lipid nanoparticles. Nanotechnology Reviews. 2013; 2(2): 201-213.




Chen WC, Zhang AX, Li SD*. Limitations and niches of the active targeting approach for nanoparticle drug delivery. European Journal of Nanomedicine. 2012; 4(2-4): 89-93.

Ernsting MJ, Murakami M, Undzys E, Aman A, Press B, Li SD*. A docetaxel-carboxymethylcellulose nanoparticle outperforms the approved taxane nanoformulation, Abraxane, in mouse tumor models with significant control of metastases. J Control Release. 2012; 162(3): 575-81.

May JP, Li SD*. Thermosensitive liposomes in cancer therapy. Recent Patents on Biomedical Engineering. 2012; 5(3): 148-158.

Tagami T, May JP, Ernsting MJ, Li SD*. A thermosensitive liposome prepared with a Cu2+ gradient demonstrates improved pharmacokinetics, drug delivery and antitumor efficacy. J Control Release. 2012; 161(1): 142-9.

Ernsting MJ, Foltz WD, Undzys E, Tagami T, Li SD*. Tumor-targeted drug delivery using MR-contrasted docetaxel-carboxymethylcellulose nanoparticles. Biomaterials. 2012; 33(15): 3931-41.

Ernsting MJ, Tang WL, MacCallum N, Li SD*. Preclinical Pharmacokinetic, Biodistribution, and Anti-Cancer Efficacy Studies of a Docetaxel-Carboxymethylcellulose Nanoparticle in Mouse Models. Biomaterials. 2012; 33(5):1445-54.




Ernsting MJ, Tang WL, MacCallum N and Li SD*. Synthetic modification of carboxymethylcellulose and use thereof to prepare a nanoparticle forming conjugate of docetaxel for enhanced cytotoxicity against cancer cells. Bioconjug Chem. 2011; 22(12):2474-86.

Tagami T, Ernsting MJ and Li SD*. Efficient Tumor Regression by a Single and Low Dose Treatment with a Novel and Enhanced Formulation of Thermosensitive Liposomal Doxorubicin. J Control Release. 2011; 152(2): 303-9.


Tagami T, Ernsting MJ and Li SD*. Optimization of a novel and improved thermosensitive liposome formulated with DPPC and a Brij surfactant using a robust in vitro system. J Control Release. 2011; 154 (3): 290-7.

Tagami T, Foltz WD, Ernsting MJ, Lee CM, Tannock IF, May JP and Li SD*. MRI monitoring of intratumoral drug delivery and prediction of the therapeutic effect with a multifunctional thermosensitive liposome. Biomaterials 2011; 32(27): 6570-6578.