Selected Publications


Wu J, Jones N, Hohenwarter L, Zhao F, Chan V, Tan Z, Carlaw T, Morin T, Li J, Kaur T, Andrew LJ, Ross C, Hedtrich S, Li SD, Systemic delivery of proteins using novel peptides via the sublingual route. J Control Release. 2024 Mar 04; ISSN 0168-3659. doi: 10.1016/j.jconrel.2024.02.042

Hohenwarter L, Puil E, Rouhollahi E, Bohrmann L, Lu S, Saatchi K, Häfeli UO, Barr A, Böttger R, Viswanadham KKD, Li SD. A Novel Leu-Enkephalin Prodrug Produces Pain-Relieving and Antidepressant EffectsMolecular pharmaceutics. 2024 Feb 5;21(2):688-703. doi: 10.1021/acs.molpharmaceut.3c00807

Wu J, Roesger S,  Jones N, Hu CM, Li SD. Cell penetrating peptides for transmucosal delivery of proteins. J Control Release. 2024 Jan; ISSN 0168-3659. doi: 10.1016/j.jconrel.2024.01.038.


Hohenwarter L, Böttger R, & Li SD. Modification and Delivery of Enkephalins for Pain ModulationInternational journal of pharmaceutics646, 123425. Epub 2023 Sep 20. doi: 10.1016/j.ijpharm.2023.123425

Wu J, Jones N, Fayez NAL, Chao PH, Wu A, de Araujo DR, Rouhollahi E, Jia A, Li SD. Protamine-mediated efficient transcellular and transmucosal delivery of proteins. J Control Release. 2023 Apr;356:373-385. doi: 10.1016/j.jconrel.2023.03.002. Epub 2023 Mar 11. PMID: 36878318 (Cover story of the Journal)


Mohammed GK, Böttger R, Krizsan A, Volke D, Mötzing M, Li SD, Knappe D, Hoffmann R. In Vitro Properties and Pharmacokinetics of Temporarily PEGylated Onc72 Prodrugs. Adv Healthc Mater. 2023 Jan 11:e2202368. doi: 10.1002/adhm.202202368. Online ahead of print. PMID: 36631971



Wang Z, Liu Y, Li S, Meng F, Yu J, Zhou S, Dong S, Ye J, Li L, Zhang C, Qiu S, Lin G, Liu H, Li SD, He Z, Wang Y. Bioinspired Nanocomplexes Comprising Phenolic Acid Derivative and Human Serum Albumin for Cancer Therapy. Nano Lett. 2022 Dec 28;22(24):10040-10048. doi: 10.1021/acs.nanolett.2c03763. Epub 2022 Dec 15. PMID: 36521033


Al Fayez N, Böttger R, Brown J, Rouhollahi E, Li SD. The Mechanism of Hepatocyte-Targeting and Safety Profile of Phospholipid-Free Small Unilamellar Vesicles. Int J Pharm. 2022 Oct 8:122269. doi: 10.1016/j.ijpharm.2022.122269. Online ahead of print. PMID: 36220590


Nguyen A, Chao PH, Ong CY, Rouhollahi E, Fayez NA, Lin L, Brown JI, Böttger R, Page B, Wong H, Li SD. Chemically engineering the drug release rate of a PEG-paclitaxel conjugate using click and steric hindrance chemistries for optimal efficacy. Biomaterials. 2022 Oct;289:121735. doi: 10.1016/j.biomaterials.2022.121735. Epub 2022 Aug 13. PMID: 36055815


Al Fayez N, Rouhollahi E, Ong CY, Wu J, Nguyen A, Roland B, Cullis PR, Witzigmann D, Li SD. Hepatocyte-targeted delivery of imiquimod reduces hepatitis B virus surface antigen. J Control Release. 2022 Sep 1;S0168-3659(22)00581-8. doi: 10.1016/j.jconrel.2022.08.058. Online ahead of print. PMID: 36058352


Al Fayez N, Böttger R, Ghosh S, Nakajima Y, Chao PH, Rouhollahi E, Nguyen A, Cullis PR, Witzigmann D, Li SD. Development of a child-friendly oral drug formulation using liposomal multilamellar vesicle technology. Int J Pharm. 2022 Aug 12;625:122107. doi: 10.1016/j.ijpharm.2022.122107. Online ahead of print. PMID: 35964828


Nguyen, Anne, Elham Rouhollahi, Roland Böttger, Chun Yat Ong, Po-Han Chao, Jiamin Wu, Yao Chen and Shyh-Dar Li. Interplay between the linker and polymer molecular weight of a self-assembling prodrug on the pharmacokinetics and therapeutic efficacy. Biomater Sci. 2022 Jun 14;10(12):3122-3136. doi: 10.1039/d1bm01947c. PMID: 35544350


Swetha KL, Maravajjala KS, Li SD, Singh MS, Roy A. Breaking the niche: multidimensional nanotherapeutics for tumor microenvironment modulation. Drug Deliv Transl Res. 2022 Jun 13. doi: 10.1007/s13346-022-01194-7. Online ahead of print. PMID: 35697894 Review.


Böttger R, Chao PH, Al Fayez N, Pauli G, Nguyen A, Hohenwarter L, Bilal N, Mohammed GK, Knappe D, Hoffmann R, Li SD. Simultaneous Chromatographic Quantitation of Drug Substance and Excipients in Nanoformulations Using a Combination of Evaporative Light Scattering and Absorbance Detectors. Mol Pharm. 2022 Jun 6;19(6):1882-1891. doi: 10.1021/acs.molpharmaceut.2c00021. Epub 2022 May 4. PMID: 35506592



Pauli, Griffin, Po-Han Chao, Zhu Qin, Roland Böttger, Suen E. Lee, and Shyh-Dar Li. Liposomal Resiquimod for Enhanced Immunotherapy of Peritoneal Metastases of Colorectal Cancer. Pharmaceutics 13, no. 10: 1696. doi: 10.3390/pharmaceutics13101696


Nojoud AL Fayez, Roland Böttger, Elham Rouhollahi, Pieter R. Cullis, Dominik Witzigmann, and Shyh-Dar Li. Improved Liver Delivery of Primaquine by Phospholipid-Free Small Unilamellar Vesicles with Reduced Hemolytic Toxicity. Molecular Pharmaceutics. doi: 10.1021/acs.molpharmaceut.1c00520


Anne Nguyen, Roland Böttger, Shyh-Dar Li. Recent trends in bioresponsive linker technologies of Prodrug-Based Self-Assembling nanomaterials. Biomaterials. doi: 10.1016/j.biomaterials.2021.120955

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K. K. DurgaRao Viswanadham, Roland Böttger, Lukas Hohenwarter, Anne Nguyen, Elham Rouhollahi, Alexander Smith,Yi-Hsuan Tsai, Yuan-Yu Chang, Christopher Llynard Ortiz, Lee-Wei Yang, Liliana Jimenez, Siyuan Li, Chan Hur, and Shyh-Dar Li. An Effective and Safe Enkephalin Analog for Antinociception. Pharmaceutics. doi: 10.3390/pharmaceutics13070927


Julian Vogler, Roland Böttger, Nojoud A.L. Fayez, Wunan Zhang, Zhu Qin, Lukas Hohenwarter, Po-Han Chao, Elham Rouhollahi, Nida Bilal, Naliangzi Chen, Brandon Lee, Christine Chen, Brayden Wilkinson, Timothy J. Kieffer, Jayesh A. Kulkarni, Pieter R. Cullis, Dominik Witzigmann, Shyh-Dar Li. Altering the Intra-Liver Distribution of Phospholipid-Free Small Unilamellar Vesicles Using Temperature-Dependent Size-Tunability. Journal of Controlled Release. doi: 10.1016/j.jconrel.2021.03.025



Anne Nguyen, Hidenori Ando, Roland Böttger, K. K. DurgaRao Viswanadham, Elham Rouhollahi, Tatsuhiro Ishida and Shyh-Dar Li. Utilization of click chemistry to study the effect of poly(ethylene)glycol molecular weight on the self-assembly of PEGylated gambogic acid nanoparticles for the treatment of rheumatoid arthritis. Biomaterials Science. doi: 10.1039/D0BM00711K









Roland Böttger, Griffin Pauli, Po-Han Chao, Nojoud AL Fayez, Lukas Hohenwarter, Shyh-Dar Li. Lipid-based nanoparticle technologies for liver targeting. Advanced Drug Delivery Reviews. doi:10.1016/j.addr.2020.06.017



Zhang, Wunan; Böttger, Roland; Qin, Zhu; Kulkarni, Jayesh; Vogler, Julian; Cullis, Pieter; Li, Shyh-Dar. Phospholipid-Free Small Unilamellar Vesicles for Drug Targeting to Cells in the Liver. Small. doi: 10.1002/smll.201901782


Hamano, Nobuhito; Böttger, Roland; Lee, Suen Ern; Yang, Yang; Kulkarni, Jayesh; Ip, Shell; Cullis, Pieter; Li, Shyh-Dar. A robust microfluidic technology and a new lipid composition for fabrication of curcumin-loaded liposomes: Effect on the anticancer activity and safety of cisplatin. Mol Pharm. 2019 Aug 5 doi: 10.1021/acs.molpharmaceut.9b00583.



Mahjub R, Jatana S, Lee SE, Qin Z, Pauli G, Soleimani M, Madadi S, Li SD. Recent advances in applying nanotechnologies for cancer immunotherapy. J Control Release. 2018 Sep 14:239-263. doi: 10.1016/j.jconrel.2018.09.010.


Agrawal M, Saraf S, Saraf S, Antimisiaris SG, Hamano N, Li SD, Chougule M, Shoyele SA, Gupta U, Ajazuddin, Alexander A. Recent advancements in the field of nanotechnology for the delivery of anti-Alzheimer drug in the brain region. Expert Opin Drug Deliv. 2018 May 17:1-29. doi: 10.1080/17425247.2018.1471058.


Tang WL, Tang WH, Li SD. Cancer theranostic applications of lipid-based nanoparticles. Drug Discov Today. 2018 May; 23(5): 1159-1166. doi: 10.1016/j.drudis.2018.04.007.


Tang WL, Tang WH, Szeitz A, Kulkarni J, Cullis P, Li SD. Systemic study of solvent-assisted active loading of gambogic acid into liposomes and its formulation optimization for improved delivery. Biomaterials. 2018 Jun; 166:13-26. doi: 10.1016/j.biomaterials.2018.03.004.


Ho L, Bokharaei M, Li SD. Current update of a thermosensitive liposomes composed of DPPC and Brij78. J Drug Target. 2018 Jan 11:1-41.



Chang CC, Yang Y, Gao DY, Cheng HT, Hoang B, Chao PH, Chen LH, Bteich J, Chiang T, Liu JY, Li SD, Chen Y. Docetaxel-carboxymethylcellulose nanoparticles ameliorate CCl4-induced hepatic fibrosis in mice. J Drug Target. 2017 Dec 27:1-9.


Hoang B, Ernsting MJ, Tang WS, Bteich J, Undzys E, Kiyota T, Li SD.Cabazitaxel-conjugated nanoparticles for docetaxel-resistant and bone metastatic prostate cancer. Cancer Lett. 2017 Dec 1;410:169-179.


Roy A, Zhao Y, Yang Y, Szeitz A, Klassen T, Li SD. Selective targeting and therapy of metastatic and multidrug resistant tumors using a long circulating podophyllotoxin nanoparticle. 2017 Aug;137:11-22. doi: 10.1016/j.biomaterials.2017.05.019


Tang WL, Tang WH, Chen WC, Diako C, Ross CF, Li SD. Development of a Rapidly Dissolvable Oral Pediatric Formulation for Mefloquine Using Liposomes. Mol Pharmaceutics. 2017 May 1 [Web]. doi: 10.1021/acs.molpharmaceut.7b00077


Hysi E, Wirtzfeld LA, May JP, Undzys E, Li SD, Kolios MC. Photoacoustic signal characterization of cancer treatment response: Correlation with changes in tumor oxygenation. Photoacoustics. 2017 Mar;5(2017):25-35.


Yang Y, Roy Aniruddha, Zhao Yucheng, Undzys E, Li SD. Comparison of Tumor Penetration of Podophyllotoxin–Carboxymethylcellulose Conjugates with Various Chemical Compositions in Tumor Spheroid Culture and In Vivo Solid Tumor. Bioconjugate Chem. 2017 Apr 24 [Web]. doi: 10.1021/acs.bioconjchem.7b00165



Yang Y, Bteich J, Li SD. Current Update of a Carboxymethylcellulose-PEG Conjugate Platform for Delivery of Insoluble Cytotoxic Agents to Tumors. AAPS J. 2016 Nov 21. [Epub ahead of print]

May JP, Hysi E, Wirtzfeld LA, Undzys E, Li SD, Kolios MC. Photoacoustic Imaging of Cancer Treatment Response: Early Detection of Therapeutic Effect from Thermosensitive Liposomes. PLoS One. 2016 Oct 27; 11(10):e0165345.


Roy A, Li SD. Modifying the tumor microenvironment using nanoparticle therapeutics. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2016 Apr 1.


Tang WL, Chen WC, Roy A, Undzys E, Li SD. A Simple and Improved Active Loading Method to Efficiently Encapsulate Staurosporine into Lipid-Based Nanoparticles for Enhanced Therapy of Multidrug Resistant Cancer. Pharm Res. 2016 May; 33(5):1104-14.


May JP, Undzys E, Roy A, Li SD. Synthesis of a Gemcitabine Prodrug for Remote Loading into Liposomes and Improved Therapeutic Effect. Bioconjug Chem. 2016 Jan 20; 27(1):226-37.



Roy A, Ernsting MJ, Undzys E, Li SD*. A highly tumor-targeted nanoparticle of podophyllotoxin penetrated tumor core and regressed multidrug resistant tumors. Biomaterials. 2015; 52:335-46.



Hoang B, Ernsting MJ, Roy A, Murakami M, Undzys E, Li SD*. Docetaxel-carboxymethylcellulose nanoparticles target cells via a SPARC and albumin dependent mechanism. Biomaterials. 2015; 59:66-76.


Zhao Y, May JP, Chen IW, Undzys E, Li SD*. A Study of Liposomal Formulations to Improve the Delivery of Aquated Cisplatin to a Multidrug Resistant Tumor. Pharm Res. 2015; 32(10):3261-8.

Ernsting MJ, Hoang B, Lohse I, Undzys E, Cao P, Do T, Gill B, Pintilie M, Hedley D, Li SD*. Targeting of metastasis-promoting tumor-associated fibroblasts and modulation of pancreatic tumor-associated stroma with a carboxymethylcellulose-docetaxel nanoparticle. J Control Release. 2015; 206:122-30.



Hoang B, Ernsting MJ, Murakami M, Undzys E, Li SD*. Docetaxel-carboxymethylcellulose nanoparticles display enhanced anti-tumor activity in murine models of castration-resistant prostate cancer. Int J Pharm 2014;471(1-2):224-33. (PDF)


Roy A, Murakami M, Ernsting MJ, Hoang B, Undzys E, Li SD*. Carboxymethylcellulose-Based and Docetaxel-Loaded Nanoparticles Circumvent P-Glycoprotein-Mediated Multidrug Resistance. Mol Pharm. 2014;11(8):2592-9 (PDF)

Roy A, Bhattacharyya M, Ernsting MJ, May JP, Li SD*. Recent progress in the development of polysaccharide conjugates of docetaxel and paclitaxel. WIREs Nanomedicine & Nanotechnology 2014; 6(4):349-68. (PDF)



May JP, Ernsting MJ, Undzys E, Li SD*. Thermosensitive liposomes for delivery of gemcitabine and oxaliplatin to tumors. Mol Pharm 2013; 10(12):4499-508.

Ernsting MJ, Murakami M, Roy A, Li SD*. Factors controlling pharmacokinetics, biodistribution and intratumoral penetration of nanoparticles. J Control Release 2013; 172(3): 782-794. (PDF)


Murakami M, Ernsting MJ, Undzys E, Holwell N, Foltz WD, Li SD*. Docetaxel conjugate nanoparticles that target α-smooth muscle actin expressing stromal cells suppress breast cancer metastasis. Cancer Research. 2013; 73(15): 4862-71. (PDF)


May JP, Li SD*. Hyperthermia induced drug targeting. Expert Opinion on Drug Delivery. 2013; 10(4): 511-27.

Chen WC, May JP, Li SD*. Immune responses of therapeutic lipid nanoparticles. Nanotechnology Reviews. 2013; 2(2): 201-213.



Chen WC, Zhang AX, Li SD*. Limitations and niches of the active targeting approach for nanoparticle drug delivery. European Journal of Nanomedicine. 2012; 4(2-4): 89-93.

Ernsting MJ, Murakami M, Undzys E, Aman A, Press B, Li SD*. A docetaxel-carboxymethylcellulose nanoparticle outperforms the approved taxane nanoformulation, Abraxane, in mouse tumor models with significant control of metastases. J Control Release. 2012; 162(3): 575-81.

May JP, Li SD*. Thermosensitive liposomes in cancer therapy. Recent Patents on Biomedical Engineering. 2012; 5(3): 148-158.

Tagami T, May JP, Ernsting MJ, Li SD*. A thermosensitive liposome prepared with a Cu2+ gradient demonstrates improved pharmacokinetics, drug delivery and antitumor efficacy. J Control Release. 2012; 161(1): 142-9.

Ernsting MJ, Foltz WD, Undzys E, Tagami T, Li SD*. Tumor-targeted drug delivery using MR-contrasted docetaxel-carboxymethylcellulose nanoparticles. Biomaterials. 2012; 33(15): 3931-41.

Ernsting MJ, Tang WL, MacCallum N, Li SD*. Preclinical Pharmacokinetic, Biodistribution, and Anti-Cancer Efficacy Studies of a Docetaxel-Carboxymethylcellulose Nanoparticle in Mouse Models. Biomaterials. 2012; 33(5):1445-54.



Ernsting MJ, Tang WL, MacCallum N and Li SD*. Synthetic modification of carboxymethylcellulose and use thereof to prepare a nanoparticle forming conjugate of docetaxel for enhanced cytotoxicity against cancer cells. Bioconjug Chem. 2011; 22(12):2474-86.

Tagami T, Ernsting MJ and Li SD*. Efficient Tumor Regression by a Single and Low Dose Treatment with a Novel and Enhanced Formulation of Thermosensitive Liposomal Doxorubicin. J Control Release. 2011; 152(2): 303-9.


Tagami T, Ernsting MJ and Li SD*. Optimization of a novel and improved thermosensitive liposome formulated with DPPC and a Brij surfactant using a robust in vitro system. J Control Release. 2011; 154 (3): 290-7.

Tagami T, Foltz WD, Ernsting MJ, Lee CM, Tannock IF, May JP and Li SD*. MRI monitoring of intratumoral drug delivery and prediction of the therapeutic effect with a multifunctional thermosensitive liposome. Biomaterials 2011; 32(27): 6570-6578.